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ldh cytotoxicity assay kit reagent  (Beyotime)


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    Beyotime ldh cytotoxicity assay kit reagent
    Ldh Cytotoxicity Assay Kit Reagent, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 5771 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cytotoxicity+assay+kits/LDH+Cytotoxicity+Assay+Kit/pmc13080478-72-31-36
    Average 99 stars, based on 5771 article reviews
    ldh cytotoxicity assay kit reagent - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: PSME2 promotes malignant progression through autophagy modulation via IL-6/STAT3 signaling pathway in esophageal squamous cell carcinoma.
    Article Snippet: Aims: This study aims to investigate the expression, clinical relevance, and functional roles of PSME2 in esophageal squamous cell carcinoma (ESCC).. Materials and methods: The biomarkers for ESCC were identified through comprehensive analysis of publicly available mRNA transcriptome data obtained from TCGA and GEO databases.. The mRNA and protein expression levels of PSME2 in ESCC were validated using two independent cohorts of ESCC samples.

    Real-time Polymerase Chain Reaction:

    Article Title: PSME2 promotes malignant progression through autophagy modulation via IL-6/STAT3 signaling pathway in esophageal squamous cell carcinoma.
    Article Snippet: Aims: This study aims to investigate the expression, clinical relevance, and functional roles of PSME2 in esophageal squamous cell carcinoma (ESCC).. Materials and methods: The biomarkers for ESCC were identified through comprehensive analysis of publicly available mRNA transcriptome data obtained from TCGA and GEO databases.. The mRNA and protein expression levels of PSME2 in ESCC were validated using two independent cohorts of ESCC samples.

    SYBR Green Assay:

    Article Title: PSME2 promotes malignant progression through autophagy modulation via IL-6/STAT3 signaling pathway in esophageal squamous cell carcinoma.
    Article Snippet: Aims: This study aims to investigate the expression, clinical relevance, and functional roles of PSME2 in esophageal squamous cell carcinoma (ESCC).. Materials and methods: The biomarkers for ESCC were identified through comprehensive analysis of publicly available mRNA transcriptome data obtained from TCGA and GEO databases.. The mRNA and protein expression levels of PSME2 in ESCC were validated using two independent cohorts of ESCC samples.

    CCK-8 Assay:

    Article Title: PSME2 promotes malignant progression through autophagy modulation via IL-6/STAT3 signaling pathway in esophageal squamous cell carcinoma.
    Article Snippet: Aims: This study aims to investigate the expression, clinical relevance, and functional roles of PSME2 in esophageal squamous cell carcinoma (ESCC).. Materials and methods: The biomarkers for ESCC were identified through comprehensive analysis of publicly available mRNA transcriptome data obtained from TCGA and GEO databases.. The mRNA and protein expression levels of PSME2 in ESCC were validated using two independent cohorts of ESCC samples.

    Article Title: Blackberry-Like Doxorubicin Loaded Hyaluronic Acid/Zinc Phthalocyanine Loaded Mesoporous Silica Nanocomposites for Long-Term Tumor Photodynamic and Chemotherapy Synergistic Therapy
    Article Snippet: .. The Cell Counting Kit-8 (CCK-8), ROS detection kits, lactate dehydrogenase (LDH) cytotoxicity assay kits (WST-8 method), and Calcein/PI cell viability and cytotoxicity assay kits were all purchased from Beyotime Ltd (Shanghai, China). .. Cleaved caspase-1 antibody (#4199) was obtained from Cell Signaling Technology (CST, Danvers, MA, USA).

    Synthesized:

    Article Title: PSME2 promotes malignant progression through autophagy modulation via IL-6/STAT3 signaling pathway in esophageal squamous cell carcinoma.
    Article Snippet: Aims: This study aims to investigate the expression, clinical relevance, and functional roles of PSME2 in esophageal squamous cell carcinoma (ESCC).. Materials and methods: The biomarkers for ESCC were identified through comprehensive analysis of publicly available mRNA transcriptome data obtained from TCGA and GEO databases.. The mRNA and protein expression levels of PSME2 in ESCC were validated using two independent cohorts of ESCC samples.

    Transfection:

    Article Title: PSME2 promotes malignant progression through autophagy modulation via IL-6/STAT3 signaling pathway in esophageal squamous cell carcinoma.
    Article Snippet: Aims: This study aims to investigate the expression, clinical relevance, and functional roles of PSME2 in esophageal squamous cell carcinoma (ESCC).. Materials and methods: The biomarkers for ESCC were identified through comprehensive analysis of publicly available mRNA transcriptome data obtained from TCGA and GEO databases.. The mRNA and protein expression levels of PSME2 in ESCC were validated using two independent cohorts of ESCC samples.

    Bicinchoninic Acid Protein Assay:

    Article Title: PSME2 promotes malignant progression through autophagy modulation via IL-6/STAT3 signaling pathway in esophageal squamous cell carcinoma.
    Article Snippet: Aims: This study aims to investigate the expression, clinical relevance, and functional roles of PSME2 in esophageal squamous cell carcinoma (ESCC).. Materials and methods: The biomarkers for ESCC were identified through comprehensive analysis of publicly available mRNA transcriptome data obtained from TCGA and GEO databases.. The mRNA and protein expression levels of PSME2 in ESCC were validated using two independent cohorts of ESCC samples.

    LDH Cytotoxicity Assay:

    Article Title: PSME2 promotes malignant progression through autophagy modulation via IL-6/STAT3 signaling pathway in esophageal squamous cell carcinoma.
    Article Snippet: Aims: This study aims to investigate the expression, clinical relevance, and functional roles of PSME2 in esophageal squamous cell carcinoma (ESCC).. Materials and methods: The biomarkers for ESCC were identified through comprehensive analysis of publicly available mRNA transcriptome data obtained from TCGA and GEO databases.. The mRNA and protein expression levels of PSME2 in ESCC were validated using two independent cohorts of ESCC samples.

    Article Title: Blackberry-Like Doxorubicin Loaded Hyaluronic Acid/Zinc Phthalocyanine Loaded Mesoporous Silica Nanocomposites for Long-Term Tumor Photodynamic and Chemotherapy Synergistic Therapy
    Article Snippet: .. The Cell Counting Kit-8 (CCK-8), ROS detection kits, lactate dehydrogenase (LDH) cytotoxicity assay kits (WST-8 method), and Calcein/PI cell viability and cytotoxicity assay kits were all purchased from Beyotime Ltd (Shanghai, China). .. Cleaved caspase-1 antibody (#4199) was obtained from Cell Signaling Technology (CST, Danvers, MA, USA).

    Cytotoxicity Assay:

    Article Title: PSME2 promotes malignant progression through autophagy modulation via IL-6/STAT3 signaling pathway in esophageal squamous cell carcinoma.
    Article Snippet: Aims: This study aims to investigate the expression, clinical relevance, and functional roles of PSME2 in esophageal squamous cell carcinoma (ESCC).. Materials and methods: The biomarkers for ESCC were identified through comprehensive analysis of publicly available mRNA transcriptome data obtained from TCGA and GEO databases.. The mRNA and protein expression levels of PSME2 in ESCC were validated using two independent cohorts of ESCC samples.

    Article Title: Deciphering the therapeutic potential and mechanisms of Artemisia argyit essential oil on flagellum-mediated Salmonella infections
    Article Snippet: Salmonellosis represents a global epidemic, and the emergence of extensively drug-resistant (XDR) Salmonella and its sustained transmission worldwide constitutes a significant public health concern.. Flagellum-mediated motility serves as a crucial virulence trait of Salmonella that guides the pathogen toward the epithelial surface, enhancing gut colonization.. Artemisia argyit essential oil, a traditional herb extract, demonstrates efficacy in treating inflammationrelated symptoms and diseases; however, its effects on flagellum assembly and expression mechanisms in anti-Salmonella activity remain inadequately explored.

    Article Title: Blackberry-Like Doxorubicin Loaded Hyaluronic Acid/Zinc Phthalocyanine Loaded Mesoporous Silica Nanocomposites for Long-Term Tumor Photodynamic and Chemotherapy Synergistic Therapy
    Article Snippet: .. The Cell Counting Kit-8 (CCK-8), ROS detection kits, lactate dehydrogenase (LDH) cytotoxicity assay kits (WST-8 method), and Calcein/PI cell viability and cytotoxicity assay kits were all purchased from Beyotime Ltd (Shanghai, China). .. Cleaved caspase-1 antibody (#4199) was obtained from Cell Signaling Technology (CST, Danvers, MA, USA).

    Article Title: Glycyrrhizic acid-loaded biomimetic hybrid liposomes targeting inflammatory cascades and PD-1/PD-L1 pathway to reverse neuroinflammation-driven cognitive decline
    Article Snippet: Lactoferrin was purchased from Macklin (Shanghai, China). .. 3,3′-Dioctadecyloxacarbocyanine perchlorate (DiO), 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR), 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI), and calcein/PI cell viability and cytotoxicity assay kits were all obtained from Beyotime Biotechnology (Shanghai, China). ..

    Article Title: Stage-matched conductive hydrogel with pH/ROS/MMP9 triple-responsive salidroside release for post-infarction myocardial repair
    Article Snippet: 2-Morpholinoethanesulfonic acid (MES, ≥99.5%) was obtained from Sigma–Aldrich (St. Louis, MO, USA), and all other chemical reagents were sourced from Aladdin Reagent (Shanghai, China). .. CCK-8, cytotoxicity assay kits, and DCFH-DA ROS detection kits were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). .. Enzyme-linked immunosorbent assay (ELISA) kits for IL-6, TNF-α, and IL-10 were obtained from Seville Biotechnology Co., Ltd. (Wuhan, China).

    Article Title: Intravesical delivery of mucoadhesive and tumor-selective-penetrating nanozymes for enhancing the PDT of bladder cancer
    Article Snippet: The samples were irradiated with an 808 nm laser at a power density of 1 W/cm2 for 3 min. For the CCK-8 assay, the medium in each well was replaced with 0.1 mL of fresh medium containing 10% CCK-8 solution and incubated for an additional 2 h. The absorbance at 450 nm was measured using a microplate reader (Agilent Bio Tek, Synergy LX, USA). .. Cell apoptosis was determined by double staining with Annexin V-FITC, a green fluorescent probe, and Propidium Iodide (PI), a red fluorescent staining solution, and analyzed using flow cytometry (Beckman Coulter, Navios, USA).Additionally, the CCK-3D and Calcein/PI cell activity and cytotoxicity assay kits from Beyotime Biotechnology Co., Ltd. (Shanghai, China) were used to evaluate the in vitro tumor inhibition effects. ..

    Article Title: Cell membrane-camouflaged nanoparticles boost combined chemo/immunotherapy for gastric cancer
    Article Snippet: Gastric cancer is a highly heterogeneous and aggressive malignancy, with conventional therapies often hampered by poor drug targeting and an immunosuppressive tumor microenvironment (TME).. To address these limitations, we developed a biomimetic nanoplatform (PLGA-UA@MA) composed of poly(lactic-co-glycolic acid) (PLGA) nanoparticles encapsulating the natural antitumor agent ursolic acid (UA), further cloaked with homologous gastric cancer cell membranes doped with the immunoadjuvant monophosphoryl lipid A (MPLA).. This engineered system synergistically combines tumor-specific targeting, immune modulation, and stimuliresponsive drug release, presenting a precision therapeutic strategy for gastric cancer.

    Article Title: CO‐Releasing Polyoxometalates Nanozyme with Gut Mucosal Immunity and Microbiota Homeostasis Remodeling Effects for Restoring Intestinal Barrier Integrity
    Article Snippet: Cell-Light EdU Apollo 567 imaging kit was obtained from Guangzhou Ribobio. .. Calcein/PI cell viability and cytotoxicity assay kits were obtained from Beyotime Biotech (China). .. Detection kits for CAT, SOD, GSH-Px,·OH, and ABTS were provided by Grace Biotech Co., Ltd. Hoechst 33342 was sourced from Keygen Biotech Co., Ltd. (Nanjing, China).

    Control:

    Article Title: Deciphering the therapeutic potential and mechanisms of Artemisia argyit essential oil on flagellum-mediated Salmonella infections
    Article Snippet: Salmonellosis represents a global epidemic, and the emergence of extensively drug-resistant (XDR) Salmonella and its sustained transmission worldwide constitutes a significant public health concern.. Flagellum-mediated motility serves as a crucial virulence trait of Salmonella that guides the pathogen toward the epithelial surface, enhancing gut colonization.. Artemisia argyit essential oil, a traditional herb extract, demonstrates efficacy in treating inflammationrelated symptoms and diseases; however, its effects on flagellum assembly and expression mechanisms in anti-Salmonella activity remain inadequately explored.

    Double Staining:

    Article Title: Intravesical delivery of mucoadhesive and tumor-selective-penetrating nanozymes for enhancing the PDT of bladder cancer
    Article Snippet: The samples were irradiated with an 808 nm laser at a power density of 1 W/cm2 for 3 min. For the CCK-8 assay, the medium in each well was replaced with 0.1 mL of fresh medium containing 10% CCK-8 solution and incubated for an additional 2 h. The absorbance at 450 nm was measured using a microplate reader (Agilent Bio Tek, Synergy LX, USA). .. Cell apoptosis was determined by double staining with Annexin V-FITC, a green fluorescent probe, and Propidium Iodide (PI), a red fluorescent staining solution, and analyzed using flow cytometry (Beckman Coulter, Navios, USA).Additionally, the CCK-3D and Calcein/PI cell activity and cytotoxicity assay kits from Beyotime Biotechnology Co., Ltd. (Shanghai, China) were used to evaluate the in vitro tumor inhibition effects. ..

    Staining:

    Article Title: Intravesical delivery of mucoadhesive and tumor-selective-penetrating nanozymes for enhancing the PDT of bladder cancer
    Article Snippet: The samples were irradiated with an 808 nm laser at a power density of 1 W/cm2 for 3 min. For the CCK-8 assay, the medium in each well was replaced with 0.1 mL of fresh medium containing 10% CCK-8 solution and incubated for an additional 2 h. The absorbance at 450 nm was measured using a microplate reader (Agilent Bio Tek, Synergy LX, USA). .. Cell apoptosis was determined by double staining with Annexin V-FITC, a green fluorescent probe, and Propidium Iodide (PI), a red fluorescent staining solution, and analyzed using flow cytometry (Beckman Coulter, Navios, USA).Additionally, the CCK-3D and Calcein/PI cell activity and cytotoxicity assay kits from Beyotime Biotechnology Co., Ltd. (Shanghai, China) were used to evaluate the in vitro tumor inhibition effects. ..

    Flow Cytometry:

    Article Title: Intravesical delivery of mucoadhesive and tumor-selective-penetrating nanozymes for enhancing the PDT of bladder cancer
    Article Snippet: The samples were irradiated with an 808 nm laser at a power density of 1 W/cm2 for 3 min. For the CCK-8 assay, the medium in each well was replaced with 0.1 mL of fresh medium containing 10% CCK-8 solution and incubated for an additional 2 h. The absorbance at 450 nm was measured using a microplate reader (Agilent Bio Tek, Synergy LX, USA). .. Cell apoptosis was determined by double staining with Annexin V-FITC, a green fluorescent probe, and Propidium Iodide (PI), a red fluorescent staining solution, and analyzed using flow cytometry (Beckman Coulter, Navios, USA).Additionally, the CCK-3D and Calcein/PI cell activity and cytotoxicity assay kits from Beyotime Biotechnology Co., Ltd. (Shanghai, China) were used to evaluate the in vitro tumor inhibition effects. ..

    Activity Assay:

    Article Title: Intravesical delivery of mucoadhesive and tumor-selective-penetrating nanozymes for enhancing the PDT of bladder cancer
    Article Snippet: The samples were irradiated with an 808 nm laser at a power density of 1 W/cm2 for 3 min. For the CCK-8 assay, the medium in each well was replaced with 0.1 mL of fresh medium containing 10% CCK-8 solution and incubated for an additional 2 h. The absorbance at 450 nm was measured using a microplate reader (Agilent Bio Tek, Synergy LX, USA). .. Cell apoptosis was determined by double staining with Annexin V-FITC, a green fluorescent probe, and Propidium Iodide (PI), a red fluorescent staining solution, and analyzed using flow cytometry (Beckman Coulter, Navios, USA).Additionally, the CCK-3D and Calcein/PI cell activity and cytotoxicity assay kits from Beyotime Biotechnology Co., Ltd. (Shanghai, China) were used to evaluate the in vitro tumor inhibition effects. ..

    In Vitro:

    Article Title: Intravesical delivery of mucoadhesive and tumor-selective-penetrating nanozymes for enhancing the PDT of bladder cancer
    Article Snippet: The samples were irradiated with an 808 nm laser at a power density of 1 W/cm2 for 3 min. For the CCK-8 assay, the medium in each well was replaced with 0.1 mL of fresh medium containing 10% CCK-8 solution and incubated for an additional 2 h. The absorbance at 450 nm was measured using a microplate reader (Agilent Bio Tek, Synergy LX, USA). .. Cell apoptosis was determined by double staining with Annexin V-FITC, a green fluorescent probe, and Propidium Iodide (PI), a red fluorescent staining solution, and analyzed using flow cytometry (Beckman Coulter, Navios, USA).Additionally, the CCK-3D and Calcein/PI cell activity and cytotoxicity assay kits from Beyotime Biotechnology Co., Ltd. (Shanghai, China) were used to evaluate the in vitro tumor inhibition effects. ..

    Inhibition:

    Article Title: Intravesical delivery of mucoadhesive and tumor-selective-penetrating nanozymes for enhancing the PDT of bladder cancer
    Article Snippet: The samples were irradiated with an 808 nm laser at a power density of 1 W/cm2 for 3 min. For the CCK-8 assay, the medium in each well was replaced with 0.1 mL of fresh medium containing 10% CCK-8 solution and incubated for an additional 2 h. The absorbance at 450 nm was measured using a microplate reader (Agilent Bio Tek, Synergy LX, USA). .. Cell apoptosis was determined by double staining with Annexin V-FITC, a green fluorescent probe, and Propidium Iodide (PI), a red fluorescent staining solution, and analyzed using flow cytometry (Beckman Coulter, Navios, USA).Additionally, the CCK-3D and Calcein/PI cell activity and cytotoxicity assay kits from Beyotime Biotechnology Co., Ltd. (Shanghai, China) were used to evaluate the in vitro tumor inhibition effects. ..

    Extraction:

    Article Title: Cell membrane-camouflaged nanoparticles boost combined chemo/immunotherapy for gastric cancer
    Article Snippet: Gastric cancer is a highly heterogeneous and aggressive malignancy, with conventional therapies often hampered by poor drug targeting and an immunosuppressive tumor microenvironment (TME).. To address these limitations, we developed a biomimetic nanoplatform (PLGA-UA@MA) composed of poly(lactic-co-glycolic acid) (PLGA) nanoparticles encapsulating the natural antitumor agent ursolic acid (UA), further cloaked with homologous gastric cancer cell membranes doped with the immunoadjuvant monophosphoryl lipid A (MPLA).. This engineered system synergistically combines tumor-specific targeting, immune modulation, and stimuliresponsive drug release, presenting a precision therapeutic strategy for gastric cancer.

    Membrane:

    Article Title: Cell membrane-camouflaged nanoparticles boost combined chemo/immunotherapy for gastric cancer
    Article Snippet: Gastric cancer is a highly heterogeneous and aggressive malignancy, with conventional therapies often hampered by poor drug targeting and an immunosuppressive tumor microenvironment (TME).. To address these limitations, we developed a biomimetic nanoplatform (PLGA-UA@MA) composed of poly(lactic-co-glycolic acid) (PLGA) nanoparticles encapsulating the natural antitumor agent ursolic acid (UA), further cloaked with homologous gastric cancer cell membranes doped with the immunoadjuvant monophosphoryl lipid A (MPLA).. This engineered system synergistically combines tumor-specific targeting, immune modulation, and stimuliresponsive drug release, presenting a precision therapeutic strategy for gastric cancer.

    MTT Assay:

    Article Title: Cell membrane-camouflaged nanoparticles boost combined chemo/immunotherapy for gastric cancer
    Article Snippet: Gastric cancer is a highly heterogeneous and aggressive malignancy, with conventional therapies often hampered by poor drug targeting and an immunosuppressive tumor microenvironment (TME).. To address these limitations, we developed a biomimetic nanoplatform (PLGA-UA@MA) composed of poly(lactic-co-glycolic acid) (PLGA) nanoparticles encapsulating the natural antitumor agent ursolic acid (UA), further cloaked with homologous gastric cancer cell membranes doped with the immunoadjuvant monophosphoryl lipid A (MPLA).. This engineered system synergistically combines tumor-specific targeting, immune modulation, and stimuliresponsive drug release, presenting a precision therapeutic strategy for gastric cancer.

    Cell Cycle Assay:

    Article Title: Cell membrane-camouflaged nanoparticles boost combined chemo/immunotherapy for gastric cancer
    Article Snippet: Gastric cancer is a highly heterogeneous and aggressive malignancy, with conventional therapies often hampered by poor drug targeting and an immunosuppressive tumor microenvironment (TME).. To address these limitations, we developed a biomimetic nanoplatform (PLGA-UA@MA) composed of poly(lactic-co-glycolic acid) (PLGA) nanoparticles encapsulating the natural antitumor agent ursolic acid (UA), further cloaked with homologous gastric cancer cell membranes doped with the immunoadjuvant monophosphoryl lipid A (MPLA).. This engineered system synergistically combines tumor-specific targeting, immune modulation, and stimuliresponsive drug release, presenting a precision therapeutic strategy for gastric cancer.



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    Image Search Results


    AGEs induce pyroptotic cell death in cardiomyocytes through the RAGE/ROS/NLRP3 pathway. Primary neonatal mouse cardiomyocytes were pretreated with or without FPS‐ZM1 (20 μ M), NAC (10 μ M), MCC950 (10 μ M), or vehicle for 1 h, followed by stimulation with AGEs (200 μ g/mL) for 24 h. (a) Representative fluorescence images of SYTOX green staining. Scale bar, 200 μ m. (b) Quantitative analysis of SYTOX‐positive cells, n = 4. (c) Representative fluorescence images of propidium iodide (PI) uptake. Scale bar, 100 μ m. (d) Quantitative analysis of PI‐positive cells, n = 4. (e) Lactate dehydrogenase (LDH) release in the cell culture supernatant was measured, n = 4.

    Journal: Journal of Diabetes Research

    Article Title: The AGEs/RAGE Axis Contributes to Myocardial Cell Death in Type 1 Diabetic Cardiomyopathy via the ROS/NF‐ κ B/NLRP3 Inflammasome Pathway

    doi: 10.1155/jdr/6438008

    Figure Lengend Snippet: AGEs induce pyroptotic cell death in cardiomyocytes through the RAGE/ROS/NLRP3 pathway. Primary neonatal mouse cardiomyocytes were pretreated with or without FPS‐ZM1 (20 μ M), NAC (10 μ M), MCC950 (10 μ M), or vehicle for 1 h, followed by stimulation with AGEs (200 μ g/mL) for 24 h. (a) Representative fluorescence images of SYTOX green staining. Scale bar, 200 μ m. (b) Quantitative analysis of SYTOX‐positive cells, n = 4. (c) Representative fluorescence images of propidium iodide (PI) uptake. Scale bar, 100 μ m. (d) Quantitative analysis of PI‐positive cells, n = 4. (e) Lactate dehydrogenase (LDH) release in the cell culture supernatant was measured, n = 4.

    Article Snippet: Trypsin solution (Cat#C0201), CM‐H2DCFDA staining (Cat#S0035S), Cy3‐conjugated secondary antibody (Cat# A0521), FITC‐conjugated secondary antibody (Cat# A0568), 4 ′ ,6‐diamidino‐2‐phenylindole (DAPI) (Cat#C1005), RIPA (Cat#P0013B), protein SDS‐PAGE protein loading buffer (5X) (Cat#P0015), BCA protein concentration measurement kit (Cat#P0011), and lactate dehydrogenase (LDH) cytotoxicity assay kit (Cat#C0016) were purchased from Beyotime (Beijing, China).

    Techniques: Fluorescence, Staining, Analysis, Cell Culture